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KMID : 0363020050350041097
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 4 p.1097 ~ p.1108
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Osteoblastic differentiation of adult stem cells by Biphasic Calcium Phosphate
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이명구
임성우/피성희/김윤상/신형식/유형근
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Abstract
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The present study was to determine the influence of micro-macro biphasic calciumphosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal
stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were
used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline
phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis
were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen
synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP
activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The
expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that
MBCP may stimulates the osteoblastic activity of ASC.2)
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KEYWORD
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Osteoblastic differentiation, mesenchymal stem cells, biphasic calcium phosphate(MBCP)
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